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Research Article

ScienceAsia 9 (1983): 143-152 |doi: 10.2306/scienceasia1513-1874.1983.09.143

 

PENICILLIN ACYLASE OF ESCHERICHIA COLI

 

VITHAYA MEEVOOTISOM and PORNPIMON SOMSUK

ABSTRACT: A new strain of Escherichia coli was isolated locally and possessed high penicillin acylase (EC 3.5.1.11) activity. The production and kinetic studies were done with the cell bound enzyme of this strain and compared to that of the reference strain, E. coli ATCC 9637. They were quite similar, in general, except that at 28 oC and with 0.064% phenylacetic acid in the growth medium the new isolate possessed higher enzyme activity per mg cell dry weight. Moreover, a mutant of this new isolate was found to possess even higher penicillin acylase activity under the same conditions. A genetic manipulation program is underway to develop this mutant for industrial use.

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Department of Microbiology. Faculty of Science Mahidol University. Rama VI Rd Bangkok 10400, Thailand

Received 17 March 1983