Volume 49 Number 3

Protective effect of high water-soluble curcuminoids on voiding dysfunction and urinary bladder hypercontractility in cyclophosphamide-induced overactive bladder in mice

Sarunnuch Sattayachiti^a, Pharkphoom Panichayupakaranant^b, Tanyarath Utaipan^c, Nipaporn Konthapakdee^a,?

 
ABSTRACT:     Overactivebladder (OAB)ischaracterized by urinary frequency, urgency and incontinence. Curcuminoids, the active compounds found in Curcuma longa Linn., exhibit anti-inflammatory, antioxidant, and antimicrobial properties. However, their clinical application is limited due to poor water solubility and low absorption. This study aimed to investigate the effects of highly water-soluble curcuminoids (HWC) formulated using a ternary inclusion complex system on voiding patterns and bladder contractile properties in OAB-induced mice. HWC were prepared using a green extraction process and a ternary inclusion complex system composed of curcuminoids, hydroxypropyl ?-cyclodextrin, and polyvinylpyrrolidone K30. Adult male mice were orally administered with HWC (50 mg/kg BW) or vehicle once daily for seven days. OAB was induced by an injection of cyclophosphamide (CYP, 150 mg/kg, i.p). CYP-treated mice showed a significant increase in the total number of urine spots, urine area, and the percentage of small urine spots. Bladders from CYP-treated group showed increased tone, amplitude, and frequency of contraction in response to carbachol, a muscarinic agonist, which was reversed with HWC administration. In summary, administration of HWC was associated with attenuation of CYP-induced alterations in voiding pattern and bladder hypercontraction through modulation of muscarinic signaling in the urinary bladder.

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* Corresponding author, E-mail: nipaporn.kon@psu.ac.th

Received 23 Feb 2026, Accepted 21 May 2026