Research articles
ScienceAsia 51 (2026): 1-9 |doi:
10.2306/scienceasia1513-1874.2026.099
Preparation of a Staphylococcus phage endolysin and its
antibacterial activity
Shihui Wang†, Yulu Wang†, Qiqi Cao, Zhenhong Yang, Qi Lin, Qiyun Guan, Xinger Lu, Bokun Lin*
ABSTRACT: A gene encoding a putative Staphylococcus aureus phage endolysin was identified in environmental
virome samples. The gene was recombinantly expressed in Escherichia coli BL21 (DE3) using the pET-30a vector,
and the resulting endolysin (designated as S2) was purified via affinity chromatography. Endolysin S2 demonstrated
lytic activity against S. aureus. Furthermore, following pre-treatment with ethylenediaminetetraacetic acid (EDTA),
endolysin S2 inhibited the growth of several Gram-negative bacteria, including Salmonella enterica serovar Enteritidis,
Salmonella enterica serovar Typhi, E. coli and Shigella. Transmission electron microscopy revealed that treatment with
endolysin S2 induced morphological distortions, cell wall damage, and a significant reduction in bacterial counts
compared to the untreated control. Biochemical characterisation indicated that endolysin S2 had a pH optimum of
8.0 and retained over 95% of its relative activity within a broad pH range of 6.0 to 10.0. The optimal temperature for
activity was 25?C, with more than 90% relative activity retained at 37?C.
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School of Public Health, Shunde Women and Children?s Hospital, Guangdong Medical University,
Guangdong 528300 China |
* Corresponding author, E-mail: bklin@gdmu.edu.cn
Received 23 Jul 2025, Accepted 1 Dec 2025
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