Research articles
ScienceAsia 51 (2023): 1-10 |doi:
10.2306/scienceasia1513-1874.2023.087
High-yield Trichoderma asperellum laccase from
microwave-LiCl composite mutagenesis and optimization
Lixia Zhua,*, Yige Maa, Wei Tana, Wenjing Maa, Jutian Chena, Lili Lib
ABSTRACT: The original strain of Trichoderma asperellum was subjected to mutagenesis using microwave irradiation
and LiCl, followed by screening for high-yield laccase mutants via the guaiacol method. Optimal fermentation
conditions were identified through single-factor and orthogonal experiments. The results indicated that a high-yield
mutant strain, MP1-1, was obtained after 15 s of microwave mutagenesis and LiCl treatment at a concentration of
0.9 g/l, achieving a lethality rate of 90%. This mutant exhibited a 70.8% increase in laccase activity compared
to the original strain. The optimized fermentation conditions were determined as follows: glucose as the carbon
source, yeast extract as the nitrogen source, pH 6.0, Cu2+ concentration of 0.09 mmol/l, inoculum volume of 1%,
and culture temperature of 28?C. Under these conditions, the laccase activity reached 54.51 U/l. After a 14-day
treatment, the weight loss rate of polyethylene treated with the mutant strain MP1-1 was 0.80%. Scanning electron
microscopy revealed significant surface erosion, characterized by pits and holes on the polyethylene, along with
the introduction of active functional groups such as carbonyl and ether bonds. The combination of microwave-LiCl
compound mutagenesis and optimized fermentation conditions significantly enhanced the laccase production capacity
of T. asperellum, providing a robust foundation for its industrial application.
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| a |
College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou 466001 China |
| b |
Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou 466001 China |
* Corresponding author, E-mail: justin2118@163.com
Received 5 May 2025, Accepted 10 Sep 2025
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