Research articles
ScienceAsia 51 (2026): 1-10 |doi:
10.2306/scienceasia1513-1874.2026.094
In vitro and in silico evaluation of α-glucosidase and
α-amylase inhibitory effects of Etlingera elatior flower
extracts and HPLC method validation for marker analysis
Weerachai Pipatrattanasereea,*, Supaluck Nuaeissarab, Sakwichai Ontongc, Aussavashai Shuayproma,
Thana Juckmetad,*
ABSTRACT: The flowers of Etlingera elatior, an edible plant belonging to the Zingiberaceae family, have been
traditionally recognized in folk medicine for their potential to reduce the risk of diabetes. In this study, we investigated
in vitro and in silico ?-glucosidase and ?-amylase inhibitory activities of various extracts from E. elatior flowers
and their major markers. Additionally, we validated an HPLC analysis. Fresh and dried E. elatior flowers were
extracted by decoction and maceration. The extracts and the two markers, chlorogenic acid and rutin, were evaluated
through ?-glucosidase and ?-amylase inhibitory assays. Molecular docking study was used to predict binding affinity
and ligand-protein interactions. A quantitative HPLC method was validated following the ICH guidelines. The
70% ethanolic extract from fresh E. elatior flowers exhibited the highest ?-glucosidase inhibitory activity (IC50
=
203.35?27.91 ?g/ml), while none of the extracts showed significant effects on ?-amylase. Both chlorogenic acid
and rutin showed potential against ?-glucosidase, but not against amylase. Rutin inhibited ?-glucosidase with a lower
IC50
value than acarbose (85.59?4.50 vs. 121.14?7.22 ?g/ml). In silico, rutin demonstrated a higher binding affinity
to ?-glucosidase than acarbose (?10.4 and ?9.0 kcal/mol, respectively). Enzyme-ligand interactions revealed that all
compounds bound to catalytic residues at the enzyme?s active site. The HPLC method for determining chlorogenic and
rutin showed good validation results. This study revealed the presence of ?-glucosidase inhibitory markers in E. elatior
f
lowers, and the validated HPLC method can be utilized for the quality control of E. elatior flower extracts.
Download PDF
Downloads Views
| a |
Regional Medical Sciences Center 12 Songkhla, Department of Medical Sciences, Ministry of Public Health,
Songkhla 90100 Thailand |
| b |
Center of Excellence on Applied Thai Traditional Medicine Research, Faculty of Medicine, Thammasat University,
Pathum Thani 12120 Thailand |
| c |
Medical Plant Research Institute, Department of Medical Sciences, Ministry of Public Health,
Nonthaburi 11000 Thailand |
| d |
Chulabhorn International College of Medicine, Thammasat University, Pathum Thani 12120 Thailand |
* Corresponding author, E-mail: weerachai.tu2557@gmail.com, thanajuckmeta9@gmail.com
Received 2 Dec 2024, Accepted 0 0000
|
