Research articles
ScienceAsia 51 (2023): 1-8 |doi:
10.2306/scienceasia1513-1874.2023.026
Cloning and expression analysis of ubiquitincarboxyl-terminal
hydrolase isozyme L5 (UCHL5) gene in Procambarus clarkii
Xian-da Bia, Jin Huanga,b, Min Penga, Xiao-han Chena,b, Yong-zhen Zhaoa, Qing-yun Liua, Qiang-yong Lia,
Peng-fei Fenga, Wei-lin Zhua, Tian-cong Chena, Xiu-li Chena, Di-gang Zenga, Yu-liu Huanga, Chun-ling Yanga,*
ABSTRACT: This study cloned the full-length cDNA sequence of Procambarus clarkii ubiquitin carboxyl-terminal
hydrolase isozyme L5 (PcUCHL5) gene using RT-PCR method. Then, the expression and subcellular localization of
the PcUCHL5 was investigated in tissues using qRT-PCR and in situ hybridization methods. The results indicated that
PcUCHL5 had a total length of 2392 bp and encoded 331 amino acids. The qRT-PCR results showed that PcUCHL5
was significantly expressed in ovarian tissue (p < 0.05), with the highest expression level in stage III ovarian tissue
during the ovarian development. Through in situ hybridization experiments, it was found that PcUCHL5 mRNA had the
strongest positive signal in primary oocytes with long-term yolk growth. The PcUCHL5 gene might play an important
role in the early ovarian development of crayfish, providing a theoretical basis for studying the regulatory mechanisms
of P. clarkii egg cell growth and development.
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| a |
Guangxi Academy of Fishery Science/Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy
Aquaculture, Nanning, Guangxi 530021 China |
| b |
College of Animal Science and Technology, Guangxi University, Nanning, Guangxi 530005 China |
* Corresponding author, E-mail: 1623952010@qq.com
Received 16 May 2024, Accepted 27 Nov 2024
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