Research articles
ScienceAsia 51 (2026): 1-10 |doi:
10.2306/scienceasia1513-1874.2026.002
Effects of MARCKS gene on skeletal muscle growth and
development in chicken and conditional knockout mice
Yingyu Luoa,b, Hua Kuia,b, Penghao Lic, Mengmeng Lia,b, Fanli Kongd, Keren Longb, Diyan Lia, Mingzhou Lib,*, Tao Wangb,*
ABSTRACT: The MARCKS gene, encoding a protein crucial for various cellular processes, was initially discovered as
a substrate for protein kinase C, a family of serine/threonine kinases involved in cell proliferation, differentiation,
and apoptosis. The function of this gene in chicken skeletal muscle growth and development is limitedly explored.
This study investigates the effects of MARCKS gene on proliferation, differentiation, and apoptosis of primary chicken
myoblasts, followed by analyzing its effects on skeletal muscle growth and development in vivo through conditional
knockout mice. Overexpression of MARCKS significantly downregulated MyoD1 and MyoG gene expression, indicating
its inhibitory role in myoblasts proliferation and differentiation. At the individual level, conditional MARCKS knockout
promoted muscle strength in mice and increased expression of MyoD1 gene in multiple skeletal tissues. These results
contribute to further understanding the function of the MARCKS gene in skeletal muscle.
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| a |
School of Pharmacy, Chengdu University, Chengdu 610106 China |
| b |
State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology,
Sichuan Agricultural University, Chengdu 611130 China |
| c |
Jinxin Research Institute for Reproductive Medicine and Genetics, Chengdu Xi Nan Gyneco-Logical
Hospital Co., Ltd., Chengdu 610000 China |
| d |
College of Life Sciences, Sichuan Agricultural University, Ya?an, Sichuan 625014 China |
* Corresponding author, E-mail: mingzhou.li@sicau.edu.cn, wangtao3@cdu.edu.cn
Received 21 Feb 2024, Accepted 0 0000
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