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Research articles

ScienceAsia (): 229-235 |doi: 10.2306/scienceasia1513-1874...229


Supplementation of sugarcane molasses for maximization of ethanol production by Saccharomyces cerevisiae using response surface method


Pongpannee Phomikheta, Wanlapa Lorliamb, Suthep Thaniyavarna, Somboon Tanasupawatc, Ancharida Savarajaraa,?

 
ABSTRACT:     Dilute sugarcane molasses containing 19.2% fermentable sugars was supplemented with (NH4)2SO4, KH 2PO4, and MgSO4 - 7 H2O to maximize the ethanol production by Saccharomyces cerevisiae TISTR 5596 using a central composite design of response surface method. The maximum ethanol (87.28 g/l) was produced when the dilute molasses was supplemented with 417 mg/l NH+ 4 , 1.88 g/l PO3- 4 and 5 mg/l Mg2+. The Ca2+ contaminated in the molasses was proposed as an indicative index for the requirement of phosphate and Mg2+ supplementation to maximize the ethanol production.

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a Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok 10330 Thailand
b Department of Microbiology, Faculty of Science, Srinakharinwirot University, Bangkok 10110 Thailand
c Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Science, Chulalongkorn University, Bangkok 10330 Thailand

* Corresponding author, E-mail: sanchari@chula.ac.th

Received 25 Mar 2018, Accepted 4 Jun 2019