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Research articles

ScienceAsia (): 141-144 |doi: 10.2306/scienceasia1513-1874...141


Detection and confirmation of hispolon in the mushroom Phellinus˙linteus


Padungrat˙Toopmuanga, Chutarat˙Khamchuma,b, Vittaya˙Punsuvona,b,c,*

 
ABSTRACT:     Phellinus linteus (PL), a mushroom known as krathin phiman in Thai, has been used as a traditional medicine in the treatment of various cancers. Hispolon obtained from PL ethanol extracts is a promising anti-cancer compound. In this study, ethanol concentration and extraction time were varied for a soxhlet extraction. The content of hispolon in a crude extract was determined by high performance liquid chromatography with a UV detector. The presence of hispolon in the crude extract was demonstrated by liquid chromatography-tandem mass spectrometry (LC-MS/MS) with daughter ion scanned mode. The results showed that both hispolon and Thai PL have a similar parent ion at m/z 218.91 and daughter ions at m/z 176, 160, 135.

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a Department˙of˙Chemistry, Faculty˙of˙Science, Kasetsart˙University, Bangkok˙10900˙Thailand
b Centre˙of˙Excellent-Oil˙Palm, Kasetsart˙University, Bangkok˙10900˙Thailand
c Centre˙of˙Advanced˙Studied˙in˙Tropical˙Natural˙Resource, NRU-KU, Kasetsart˙University, Bangkok˙10900˙Thailand

* Corresponding author, E-mail: fscivit@ku.ac.th

Received 6 Aug 2013, Accepted 7 Jan 2014