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In vitro sterilization and shoot induction of durian cv. Chani (Durio zibethinus Murr.) using chlorine dioxide and plant growth regulators

Sompong Te-chato^a, Waraporn Heedchim^b, Chakriya Niha^a, Sureerat Yenchon^a,*

 
ABSTRACT:     Tissue culture of durian remains a bottleneck problem due to difficulty in surface sterilization and regeneration of initial explants caused by mucilage secretion after explant wounding. Chlorine dioxide (ClO2 ) is a disinfectant and can be used for plant tissue culture instead of the autoclaving medium. The aim of this research was to study effects of ClO2 and plant growth regulators (PGRs) on in vitro sterilization and shoot induction. Ex vitro nodal segment was cut and directly transferred to the medium with different concentrations of ClO2 compared to the autoclaving medium. The results showed that 90 mg/l ClO2 gave the best results in sterilization at 45.45% and axillary bud break at 60%. For concentrations of N6-benzyladenine (BA) tested, the highest axillary bud break at 71.42% was obtained from 1 mg/l BA-containing medium after 4 weeks of culture. When transferring sterile axillary shoot to gibberellic acid (GA3)-containing culture medium of different types, the results demonstrated that shoot elongation was successful from culturing on solid medium overlaid with liquid medium with the same components together with 1 mg/l GA3 for 4 weeks. It can be concluded that ClO2 improves sterilization of culture medium and explants. BA together with GA3 promote shoot development of durian cv. Chani.

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* Corresponding author, E-mail: sureerat.y@psu.ac.th

Received 20 Mar 2024, Accepted 0 0000