Research articles
ScienceAsia 49 (2023):ID 603-609 |doi:
10.2306/scienceasia1513-1874.2023.061
Biological activity and alkaloid compounds of Mahonia
napaulensis DC. extracts
Jinfeng Yanga,?, Nguyen TLAb,?, Myong Jo Kimb,*, Eun Soo Seongb, Yong Soo Kwonc, Nam Ho Yood
ABSTRACT: Mahonia napaulensis DC. has historically been used for traditional medicine in Vietnam. However, the
biological mechanisms underlying its medicinal effects are not yet fully understood. In the present study, a crude extract
derived from the M. napaulensis stem was fractionated with n-hexane, ethyl acetate (EtOAc), and water-saturated
butanol (BuOH). The antioxidant activities of the crude extract and the fractionated samples were evaluated using
a DPPH assay and a reducing power assay. Our results revealed that the EtOAc fraction showed the highest total
phenolic content as well as the highest free radical scavenging activity and reducing power. The antiproliferative
effects of M. napaulensis DC. extracts were detected using a MTT assay. The results showed a significant reduction in
cell viability of over 70% in human colon carcinoma CoLo 205 cells at the highest concentration tested (200 ?g/ml). We
also used quantitative reverse transcription-polymerase chain reaction (RT-PCR) to measure the expression of mRNA
transcripts involved in the regulation of cell proliferation such as survivin, p53, caspase-3, and caspase-9 in CoLo 205
cells treated with the crude extract and fractions to better understand the changes induced by the BuOH fraction. Two
alkaloid compounds that possess strong antiproliferative activities in colon cancer cells, berberine and palmatine, were
isolated from the BuOH fraction. The results from this study indicate that M. napaulensis may be considered a medicinal
plant due to its anti-oxidative and antiproliferative properties.
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a |
Research Institute of Food Science & Engineering Technology, Hezhou University, Hezhou 542899, Guangxi, China |
b |
Bioherb Research Institute, Kangwon National University, Chuncheon 24341 Republic of Korea |
c |
Department of Pharmacy, Kangwon National University, Chuncheon 24341 Republic of Korea |
d |
Hankook Cosmetics Manufacturing Co., Ltd., Seoul 03188 Republic of Korea |
* Corresponding author, E-mail: kimmjo@kangwon.ac.kr
Received 27 May 2022, Accepted 8 Apr 2023
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