Research articles
ScienceAsia 49 (2023):ID 485-490 |doi:
10.2306/scienceasia1513-1874.2023.038
Study of Streptococcus pneumoniae serotype 19A blood
isolates from Thailand by enterobacterial repetitive intergenic
consensus (ERIC)-PCR typing
Thitiya Yungyuena, Piriyaporn Chongtrakoola, Wanatpreeya Phongsamartb, Unchalee Puangprasarta, Chalermsri Pummangurac, Dararat Samretwitc, Somporn Srifuengfungc,*
ABSTRACT: Streptococcus pneumoniae serotype 19A is frequently isolated worldwide. In this study, the clonal
relationships among 62 isolates from different patients from 21 hospitals between 2008 and 2018 were characterized
using enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR). The different band
patterns that appeared upon agarose gel electrophoresis were used to construct an unweighted pair group method
with an arithmetic mean (UPGMA) dendrogram. There were 23 different ERIC types (E1?E23). The most prevalent
type was E9, accounting for 19.36% of all isolates, followed by E6 at 16.13%, E5 at 11.30%, and 13 ERIC types
present in only one isolate or at 1.61% each. Using an additional study to determine the clonal relationships, we
compared our ERIC-PCR results to the corresponding multilocus sequence types (MLSTs) from our recent study with
the same 62 S. pneumoniae serotype 19A isolates. The results showed there were 20 different MLST types and that
ERIC-PCR was comparable to MLST as a valuable complementary tool for the investigation of S. pneumoniae serotype
19A isolates. Furthermore, ERIC-PCR is very fast, affordable, and easy to perform compared to MLST. However, there
is less concordance between these two methods. These results suggest a high diversity of different ERIC-PCR and MLST
patterns. Overall, the combination of results from both methods can add greater discrimination and complementary
information for the differentiation of S. pneumoniae strains in Thailand.
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a |
Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700 Thailand |
b |
Department of Pediatrics, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700 Thailand |
c |
Faculty of Pharmacy, Siam University, Bangkok 10160 Thailand |
* Corresponding author, E-mail: somporn.sri@mahidol.ac.th
Received 5 May 2022, Accepted 19 Mar 2023
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