Research articles
ScienceAsia 48 (2022):ID 827-832 |doi:
10.2306/scienceasia1513-1874.2022.123
Effect of diosgenin in the inhibition of gastric cancer cell
proliferation and its mechanism in combination with TRAIL to
induce apoptosis
Qingwei Zhanga,†, Sheng Zhengb,†, Xuelian Baic, Chuntian Mad, Yuqian Qie,*
ABSTRACT: Clinical observations suggest that identifying novel curative and preventive approaches by targeting the
tumor cells without affecting the normal cells is appreciable. Diosgenin (DG) is a natural sapogenin that has been
reported of having pro-apoptotic and anti-cancer properties against various neoplasia. Hence, the present study
investigated the effect of combined DG/TNF-related apoptosis-inducing ligand (TRAIL) on gastric cancer cell lines
(BGC-823) in vitro. Cell viability and IC50 for DG and TRAIL alone or in combination were determined using MTT. The
apoptosis rate was assessed by ELISA cell death assay and Caspase 8 activity assays. The gene expression evaluation
of candidate genes, including survivin, Bcl-2, XIAP, c-IAP1, c-IAP2, and c-FLIP, were accomplished before and after
the treatment by quantitative real-time PCR. Our results demonstrated that DG synergistically enhanced the cytotoxic
effects of TRAIL (p < 0.01). DG could exaggerate cell apoptosis through TRAIL-induced apoptosis and amplify the
Caspase 8 activity. These results were confirmed by decreasing anti-apoptotic genes? expression levels. Overall, our
findings shed light on a novel strategy of TRAIL-induced apoptosis in combination with DG for the treatment of gastric
cancer.
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* Corresponding author, E-mail: qiyuqian147@gmail.co
Received 16 Dec 2021, Accepted 10 Mar 2022
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