Research articles
ScienceAsia 47 (2021):ID 287-292 |doi:
10.2306/scienceasia1513-1874.2021.030
Serum carbonic anhydrase combined with adiponectin
as biomarkers of insulin resistance
Kanyanath Piumngama,*, Pilaiwan Siriprungponga, Sittiruk Roytrakulb
ABSTRACT: Insulin resistance is the main cause of type 2 diabetes. The hyperinsulinemic-euglycemic clamp is a gold
standard method for determination of the insulin resistance. However, the test cannot directly measure the insulin
resistance, and it is time consuming, costly, labor-intensive and requires an experienced operator. This study aims to
develop the protein marker for insulin resistance. Two serum proteins, i.e. carbonic anhydrase and adiponectin were
determined by using sandwich enzyme-linked immunosorbent assay (ELISA) in 3 groups of volunteers’ sera: healthy
group (n = 35), insulin resistance group (n = 32) and diabetic group (n = 32). We explored the associations between
these proteins and the insulin resistance. Adiponectin (cutoff ≤ 4.73 µg/ml, area under the curve [AUC] = 0.644
[0.51, 0.777]) (p < 0.05) and carbonic anhydrase (cutoff ≥ 47 pg/ml, AUC = 0.65 [0.518, 0.783] (p < 0.050) have
the potential as the biomarkers for diabetes. When used together, adiponectin at cutoff level ≤ 4.96 µg/ml and carbonic
anhydrase at cutoff level ≥ 36 pg/ml have the potential as the biomarkers for insulin resistance (AUC = 0.664 [0.532,
0.796]) (p < 0.05). These serum protein levels will be a useful tool to diagnose the insulin resistance in human and to
guide the right direction of treatment and the behavior change accordingly.
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| a |
Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University,
Pathum Thani 12121 Thailand |
| b |
Genome Institute, National Center for Genetic Engineering and Biotechnology, Pathum Thani 12121
Thailand |
* Corresponding author, E-mail: kanyanath.p@allied.tu.ac.th
Received 27 Oct 2020, Accepted 24 Jan 2021
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