ScienceAsia 51 (2025):ID 2026008 1-11 |doi:
10.2306/scienceasia1513-1874.2026.008
Near-infrared fluorescent probes for super-resolution imaging
Guoyong Liua, Yen Leng Pakb, Wenxiu Wub, Yurong Guob, Zhao Wangb, Yilin Fuc, Yingying Jingb,*, Danying Lind,*
ABSTRACT: Duringthepasttwodecades, super-resolution microscopies (SRMs) have become powerful tools to resolve
subcellular structures in a range of 10 nm to 100 nm. Progress in developing fluorescent probes is indispensable for the
advancements in SRMs. However, it remains challenging to recognize and observe the bio-complexes in deep imaging
depth with high resolution. The emergence of near-infrared (NIR) fluorescent probes brings new light for super
resolution fluorescent imaging due to less light scattering, decreased background signals of auto-fluorescence and low
phototoxicity. Thus, in recent years, significant efforts have been made in the development of NIR fluorescence probes
for visualizing the fine structures and organizations in cells with high resolution and excellent signal-to-noise ratio.
Here we reviewed the recent progress in NIR probes that have been successfully applied to SRMs, including fluorescent
proteins, molecular probes, and fluorescent nanomaterials. We also provided a potential prospect on the requirements
in future development of NIR fluorescent probes for SRM applications.
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| a |
Shanghai Key Laboratory of Molecular Imaging, Shanghai University of Medicine and Health Sciences,
Shanghai 201318 China |
| b |
College of Biological and Chemical Engineering, Qilu Institute of Technology, Jinan 250200 China |
| c |
Department of Thoracic Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University,
Jinan 250021 China |
| d |
Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College
of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060 China |
* Corresponding author, E-mail: yyjng@mail.ustc.edu.cn, dylin@szu.edu.cn
Received 22 Aug 2025, Accepted 24 Dec 2025
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