 BackResearch Article ScienceAsia 24 (1998): 241-250 |doi: 10.2306/scienceasia1513-1874.1998.24.241 SOMATIC EMBRYOGENESIS FROM CELL SUSPENSION CULTURES OF OIL PALM (ELAEIS GUINEENSIS JACQ.)KAMNOON KANCHANAPOOM AND BOONSANONG CHOURYKAEWABSTRACT: Zygotic embryos of oil palm (Elaeis guineensis jacq.) were excised and cultured on callus induction medium (Eeuwens, 1976i 1978) containing 2 mg/l 2A-dichlorophenoxyacetic acid (2A-D). Two months-old calli derived from embryos were transferred to liquid callus induction medium without 2,4-D to establish fine suspension cultures. The establishment of cell cultures were obtained in 2-3 months. Cell suspensions were then subcultured to several regeneration media and embryoid differentiation occurred either in regeneration medium supplemented with 20% (v/v) coconut water or 0.01 mg/l a Department of Biology, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkla 90112, Thailand. Received September 25, 1998
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-naphthaleneacetic acid (NM). However, no plantlet development was recovered. These embryoids were fixed, sectioned, stained and examined microscopically. The results revealed their bipolar structure consisted of shoot and root meristem, procambial cells and protodermal layer.
