 BackResearch Article ScienceAsia 22 (1996): 013-020 |doi: 10.2306/scienceasia1513-1874.1996.22.013 SOMATIC EMBRYOGENESIS AND PIANTLET REGENERATION FROM OIL PALM (ELAEIS GUINEENSIS JACQ.) CALLUSWISUT PATCHARAPISUTSIN AND KAMNOON KANCHANAPOOMABSTRACT: Callus cultures were initiated from mature embryos of oil palm (Elaeis guineensis Jacq.) on half strength MS (Murashige and Skoog, 1962) medium enriched with either 30 mg/l NAA ( Department of Biology, Faculty of Science, Prince of Songkla University, Haad-Yai, Songkla 90112, Thailand. Received May 8, 1995
|
-naphthaleneacetic acid) or 3 mg/l 2,4-D (2,4-dichlorophenoxyacetic acid), 3% sucrose and 0.05% (w/v) activated charcoal. Induction of embryogenic, fast-growing calli were obtained when calli were transferred to similar medium with an increase of NAA and 2,4-D to 70 mg/l and 5 mg/l, respectively. These embryogenic calli produced numerous embryoids in these media after several subcultures. Somatic embryo maturation was obtained on medium devoid of NAA or 2,4-D and supplemented with 15% (v/v) coconut water to produce young plantlets. Histological study of embryoids at different stages of development revealed the morphological organization resembled that of zygotic embryo.
