ScienceAsia 17 (1991): 095-102 |doi: 10.2306/scienceasia1513-1874.1991.17.095

ORIGIN AND DEVELOPMENT OF CALLUS FORMATION FROM CULTURED BUD OF VANDA ORCHID

KAMNOON KANCHANAPOOM^a, THAVORN VAJRABHAYA^b AND OBCHANT THAITONG^b

ABSTRACT: Lateral buds of Vanda x Miss Joaquim were cultured on a modified agar medium containing 10% coconut water and 0.1 mg/l -naphthalene acetic add for one month before being transferred to a liquid medium. Histological analysis was carried out on paraffin sections stained with hematoxyIin. The cultured buds on an agar medium resembled a typical angiosperm shoot apex. After leaf excision, some of these buds initiated meristematic cells on the abaxial position of young leaves. Serial sections revealed that callus formation originated from a single meristematic cell that had dense cytoplasm with a large nucleus. The calli were composed of parenchyma cells bounded by an epidermis-like layer and subsequently these cells differentiated into protocorm-like bodies when they were submerged in a liquid medium.

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a Department of Biology, Faculty of Sdence, Prince of Songkla University, Hat-Yai, Songkla 90112, Thailand.
b Department of Botany, Faculty of Sdence, Chulalongkom University, Bangkok 10500, Thailand.

Received 7 June 1991