 BackResearch Article ScienceAsia 16 (1990): 017-024 |doi: 10.2306/scienceasia1513-1874.1990.16.017
SIMPLE IN VITRO CULTURE OF EMBRYOS OF THE GIANT FRESHWATER PRAWN (MACROBRACHIUM ROSENBERGII)
PRANEET DAMRONGPHOL, NITTAYA EANGCHUAN AND BOONSERM POOL-SANGUANABSTRACT: Embryos of the giant freshwater prawn were cultured in vitro in deionized water and in 15%,30% and 45% artificial seawater (ASW), respectively. Deionized water and 45% ASW did not support development to hatching whereas 15% and 30% ASW supported normal development and provided 25.6% :t 2.3 and 27.1% :t 1.2 hatching rates on day 18.5, respectively. Addition of 100, 200 or 300 pg/ml streptomycin to the 15% or 30% ASW did not improve the survival or hatching rate. On. the other hand, the presence of 0.3 part per million (ppm) formalin in 15% ASW improved the hatching rate to 47.1 :t 6.1 on day 18.5. Higher formalin levels of 0.6 or 0.9 ppm were detrimental to the developing embryos. Department of Biology, Faculty of Science, Mahidol University, Rama VI Road, Bangkok 10400, Thailand.
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