ScienceAsia 5 (1979): 161-167 |doi: 10.2306/scienceasia1513-1874.1979.05.161

EFFECT OF CHEMICAL MODIFICATION OF N-ACETYLNEURAMINIC ACID ON HORMONAL ACTIVITY OF ERYTHROPOIETIN IN VIVO AND IN VITRO

PRASONG KOONANUWATCHAIDET^a, BOONSONG WINYAR^b and MAITREE SUTTAJIT^b

Summary: Sheep, rabbit and human erythropoietins (EP) were oxidized by periodate and then reduced by potassium borohydride, yielding EP containing analogues of N-acetyl-neuraminic acid (NANA) shortened by 1 or 2 carbon atoms. The EP activity of the modified preparations was assayed in vivo by ⁵⁹Fe incorporation in starved rats and in vitro incorporated in bone marrow cells. The modification enhanced the in vivo activity by 2-3 times, possibly due to an increased resistance of the hormone to endogenous neuraminidase. However, the in vitro activity of the modified EP was reduced by about 20 percent from native preparations.

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a Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
b Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

Received 22 October 1979