ScienceAsia 3 (1977): 034-041 |doi: 10.2306/scienceasia1513-1874.1977.03.034

EFFECTS OF HEAT AND SOLVENTS ON THE CIRCULAR DICHROISM OF DISUCCINYLATED GLUCAGON

BHINYO PANIJPAN

Summary: Disuccinylated glucagon was studied by circular dichroism, at different temperatures, In the presence of urea, lithium chloride and three organic solvents. Ethanol, 2-chloroethanol and 2,2,2-trifluoroethanol. in order of effectiveness, promoted helix formation. Urea enhanced the positive band at 227 nm, whereas lithium chloride caused an increase in the negative band at 222 nm. Increase in temperature caused a negative inversion of ()_{230 nm} in high urea (and guanidine hydrochloride) concentrations and a positive ()_{230 nm} nm inversion in lithium chloride. Results obtained with glucagon are used for comparison.

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Department of Biochemistry, Faculty of Science, Mahidol University, Rama VI Road, Bangkok, Thailand

Received 31 January 1977