Physicochemical and microbiological property of disclosing agent for biofilm detection: An in-vitro study

Panita Prommoola, Nattapon Rotpenpian

ABSTRACT: This study aimed to evaluate the efficacy of the recently developed Ezy Gel D in identifying pathogenic characteristics of plaque, crucial for predicting the risk of caries. Distinct features of Ezy Gel D were explored, including its purple appearance and color transformation upon interaction with Streptococcus mutans biofilm. Acid production effects, viscosity, and pH stability were assessed over 60 min. Microbiological quality testing was conducted on day 1 and day 180. Ezy Gel D exhibited a notable purple-to-deep-blue color transformation interacting with S. mutans biofilm but showed no changes with acid production. The viscosity remained stable (low to medium), and pH values consistently ranged between 8.02 and 8.04. Microbiological testing on day 1 and day 180 revealed no issues, affirming product stability. The study confirms Ezy Gel D as a dependable and stable product for dental applications. Its favorable physical and chemical characteristics, coupled with the absence of microbiological issues, highlight its potential suitability for various dental procedures.

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^a	Dental Hospital, Faculty of Dentistry, Prince of Songkla University, Songkhla 90110 Thailand
^b	Department of Oral Biology and Occlusion, Faculty of Dentistry, Prince of Songkla University, Songkhla 90110 Thailand

* Corresponding author, E-mail: nattapon.r@psu.ac.th

Received 7 Jun 2024, Accepted 30 Nov 2024