Research articles
ScienceAsia 51 (2023): 1-9 |doi:
10.2306/scienceasia1513-1874.2023.011
Antibacterial activity of silver nanoparticles against Xanthomonas compestris var. compestris
Meng Li, Tianya Lei, Jiaxuan Li, Xinyu Li, Zhenlin Wei*
ABSTRACT: Cabbage black rot, a disease caused by Xanthomonas compestris var. compestris (Xcc), has significantly
impeded the growth and development of Chinese cabbage. In this research, silver nanoparticles (AgNPs) were
synthesized using Solidago canadensis as the primary material, and their capacity to inhibit the growth of black
rot pathogens was evaluated. The results indicated that the AgNPs exhibited a spherical morphology with an
average particle size of 10.22 nm. The minimum inhibitory concentration (MIC) and the minimum bactericidal
concentration (MBC) against Xcc were determined to be 10 ?g/ml. In particular, the inhibition circle assay and
growth curve evaluation showed a profound suppressive effect of AgNPs on bacterial proliferation. Furthermore,
treatment with AgNPs resulted in structural damage to bacterial cells, a reduction in hydrogen peroxide tolerance,
and significantly hindered biofilm development. Synergistic evaluations with Zhongshengmycin, an aminoglycoside
antibiotic, highlighted a significant collaborative effect in enhancing resistance to black rot with a Fractional Inhibitory
Concentration (FIC) index of 0.335. These in vitro results showed that the combined application of 1.25 ?g/ml AgNPs
and 0.25 ?g/mlZhongshengmycincould inhibit pathogen infestation on explants and thus significantly reduce the dose
of antibiotics in production. This study can potentially provide novel insights into preventing and treating cabbage black
rot.
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College of Life Sciences, Dezhou University, Shandong 253023 China |
* Corresponding author, E-mail: wzl19741028@163.com
Received 14 Apr 2024, Accepted 13 Oct 2024
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