Research articles
ScienceAsia 51 (2023): 1-8 |doi:
10.2306/scienceasia1513-1874.2023.013
Optimization of suspension culture of adapted baby hamster kidney cells in a bioreactor for maximum cell density
Afifa Ghafoora, Imran Altafa,*, Aftab Ahmad Anjuma, Ali Raza Awanb
ABSTRACT: Thebabyhamsterkidney-21 (BHK-21) cell line is a continuous anchorage-dependent cell line. Suspension
culture technology enables rapid growth of BHK-21 cells, leading to large-scale and cost-effective production of
vaccines. This study aimed to optimize suspension culture of BHK-21 cells to achieve the maximum cell density in
a bioreactor. BHK-21 cells were optimized for certain physicochemical factors like seeding ratio, pH, agitation speed,
serum concentration, medium composition, and temperature in a bioreactor. Here, this study reports the successful
adaptation of adherent BHK-21 cells to grow in suspension to a viable cell density of 7.5?106 cells/ml on the 3rd
day in self-formulated cell culture medium at pH 7.2 and 37?C, agitation speed of 50 rpm, seeding density of 4?105
cells/ml, and 10% serum concentration in a 5-l bioreactor. The BHK-21 cell suspension culture was expanded to a 5-l
bioreactor from a 20-ml flask, which resulted in the maximum cell density. The suspension culture technology improved
the maximum cell density of BHK-21 cells in the bioreactor, indicating its potential for foot and mouth disease (FMD)
vaccine production.
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Institute of Microbiology, University of Veterinary and Animal Sciences, Lahore, Punjab, Pakistan |
| b |
Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Punjab, Pakistan |
* Corresponding author, E-mail: imran.altaf@uvas.edu.pk
Received 3 Apr 2024, Accepted 24 Dec 2024
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