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Volume 49 Number 3

Research articles

ScienceAsia 49 (2023): 290-296 |doi: 10.2306/scienceasia1513-1874.2023.016

Identification of key odor compounds in the burnt smell of upper tobacco leaves through the molecular sensory science technique

Ruili Lia, Kang Chena, Liqun Lib,*, Saiyue Zhaob, Chunsheng Guob, Xinhui Wanga, Junsong Zhanga, Miao Lianga,*

ABSTRACT:     Silica gel column chromatography, gas chromatography-olfactometry (GC-O) combined with GC-mass spectrometry (GC-MS) and recombination-omission tests led to the identification of the key odor compounds in the burnt smell of upper tobacco leaves. The results showed that a total of 16 phenolic compounds were identified from the extracts of the burnt smell components (EBSC), and 10 of which were identified as important odorants in EBSC with odor activity values (OAVs) greater than 1. In addition, the recombination tests showed that there was no significant difference in the five odors attributes between the original EBSC and the recombinant model, proving the successful simulation of the typical odors of EBSC. The omission tests demonstrated that the key odor compounds in EBSC (p < 0.05) were o-cresol, p-cresol, 2,6-dimethylphenol, 2-ethylphenol, 2,4-dimethylphenol, 4-ethylphenol, 3,5- dimethylphenol, and 2,4,6-trimethylphenol. Further studies on characterization of key odor compounds in EBSC will be helpful to the directional elimination of the burnt smell from upper tobacco leaves.

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a College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, Henan 450001 China
b Inner Mongolia Kunming Cigarette Co., Ltd., Hohhot, Inner Mongolia 010020 China

* Corresponding author, E-mail: li867057788@163.com, liangmiaozzu@163.com

Received 6 Apr 2022, Accepted 6 Dec 2022