ScienceAsia 49 (2023): 1-7 |doi:
Extracorporeal shock wave protects chondrocytes against
interleukin-1β-induced apoptosis and promotes autophagy
Huanhuan Wanga, Peng Tangb, Hongwei Mina,b,*
ABSTRACT: Osteoarthritis (OA) is a common joint disease, but there is no effective treatment except surgery at present.
Extracorporeal shock wave (ESW) is an emerging therapy widely used in various medical fields. In this study, we
mainly investigated the effects of ESW on apoptosis and autophagy of osteoarthritic chondrocytes by analyzing the
cell apoptosis and the expression of autophagy markers? mRNAs and proteins, respectively. Primary chondrocytes were
isolated from articular cartilage tissue of rats. Results showed that ESW effectively inhibited the interleukin-1?-induced
(IL-1?-induced) chondrocyte apoptosis. Moreover, ESW treatment elevated the mRNA and the protein expression levels
of Beclin 1, Atg5, LC3B, and Collagen II. Beclin 1 plays a key role in autophagy, positively regulating autophagic activity.
Atg5 is a marker of autophagic activity, and LC3 is a specific marker of autophagosome. Collagen II is an important
indicator to judge the functional activity of chondrocytes. In contrast, the mRNA and the protein expression levels of
P62, which is a measure of autophagic flux and is thought to be negatively correlated with autophagic degradation,
were decreased in the ESW treated cells. This study reveals the role of ESW in promoting chondrocyte autophagy
and suppressing cell apoptosis. Thus, ESW may protect chondrocytes against IL-1?-induced apoptosis and promote
autophagy in an in vitro model of osteoarthritis.
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||Faculty of Rehabilitation Medicine, Capital Medical University, Beijing 100054 China
||Department of Orthopedics and Rehabilitation, Beijing Bo?ai Hospital, China Rehabilitation Research Center,
Beijing 100068 China
* Corresponding author, E-mail: firstname.lastname@example.org
Received 14 Sep 2021, Accepted 5 May 2022