Research articles
ScienceAsia 51 (2023): 1-8 |doi:
10.2306/scienceasia1513-1874.2023.043
Co-immobilization of levansucrase and endo-levanase on
Immobead 150P support for fructooligosaccharide production
Thanapon Charoenwongpaiboona,*, Karan Wangpaiboonb,c, Pawinee Panpetchb,c, Rath Pichyangkurab
ABSTRACT: Fructooligosaccharides (FOS) are commonly used as alternative sweeteners in healthy food and beverages
due to their zero-calorie content and biologically beneficial activity. In this study, levan-type FOS were synthesized
using co-immobilized levansucrase and endo-levanase. The enzymes were co-immobilized on Immobead 150P, which
was activated with ethylenediamine and glutaraldehyde. The effects of immobilization time and enzyme concentration
on immobilization efficiency and enzyme activity were investigated. Biochemical characterization revealed that the
optimal pH and temperature for catalysis by both enzymes remained unchanged after immobilization. Moreover, the
thermostability of both enzymes was significantly improved. FOS synthesis by the immobilized enzymes was examined
using sucrose syrup as a substrate. The results showed that the immobilized enzymes could synthesize FOS with a yield
of ?45% across a broad range of pH (pH 3?7) and temperature (30?40?C), indicating their potential for industrial
applications.
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| a |
Department of Chemistry, Faculty of Science, Silpakorn University, Nakhon Pathom 73000 Thailand |
| b |
Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330 Thailand |
| c |
Center of Excellence in Structural and Computational Biology, Department of Biochemistry, Faculty of Science,
Chulalongkorn University, Bangkok 10330 Thailand |
* Corresponding author, E-mail: charoenwongpaib_t@su.ac.th
Received 14 Dec 2024, Accepted 21 Apr 2025
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