Research articles
ScienceAsia 51 (2023): 1-8 |doi:
10.2306/scienceasia1513-1874.2023.083
Tangeretin protects mice from sepsis-induced myocardial
injury by inhibiting TRIM8 expression through upregulation
of miR-20a-5p
Ji Shi, QiYin Sun*
ABSTRACT: In this study, tangeretin (TAN), a plant-derived flavonoid, was investigated for its role in sepsis-induced
myocardial injury (SIMI). C57BL/6 mice were gavaged daily with 50 mg/kg TAN, while lentiviruses interfering with
miR-20a-5p or TRIM8 expression were injected into the tail vein for three consecutive days. Then, a mouse model
of sepsis was established by cecum ligation puncture (CLP). Levels of serum myocardial injury markers (CK-MB and
cTnI) were detected by a fully automated analyzer. The histopathology of mouse myocardium was observed by HE
staining. Apoptosis was detected by TUNEL staining in mouse myocardial tissues. TAN improved cardiac dysfunction
and myocardial injury in CLP mice. TAN could upregulate miR-20a-5p and downregulate TRIM8 levels. The beneficial
effects of TAN could be attenuated by downregulating miR-20a-5p or upregulating TRIM8. TAN protects mice from
SIMI by inhibiting TRIM8 expression through upregulation of miR-20a-5p.
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Department of Cardiology, Huzhou First People?s Hospital, Zhejiang 313000 China |
* Corresponding author, E-mail: dr.sunqiyin@outlook.com
Received 10 Oct 2024, Accepted 0 0000
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