Research articles
ScienceAsia 51 (2023): 1-10 |doi:
10.2306/scienceasia1513-1874.2023.028
Caspase-1 inhibitor VX-765 alleviates PM2.5-induced hepatic
lipid metabolism disorder in mice
Sisi Qina, Lei Wub, Yongheng Wanga,c, Ning Lia, Changyu Qina, Shoufang Jianga, Jianfen Weid,
Naijun Wud, Fuyuan Caoa,e,*, Shuang Lia,c,e,*
ABSTRACT: Long-term exposure to PM2.5 is associated with hepatic lipid metabolism disorders, in which caspase-1 is
involved, but the mechanism is not fully understood. This study focused on the role of the caspase-1 inhibitor VX-765.
Along-term PM2.5 exposure model was constructed by concentrating ambient air particles. Mice were intraperitoneally
administered with VX-765. The body weight, liver index, blood lipid, and hepatic disorder indices were measured.
Hematoxylin and eosin staining, immunohistochemical staining, Western blot analysis, and real-time quantitative
polymerase chain reaction were employed to assess histopathological changes and quantify the expression levels of
target proteins and mRNAs. The results indicated that VX-765 could inhibit hepatic disorder, improve body weight and
liver index, and regulate lipid metabolism by affecting the expression of key factors such as caspase-1, IL-1?, SREBP-1,
PPAR-?, PPAR-?, and PDK4. In conclusion, caspase-1 inhibitors offer a novel approach for alleviating hepatic disorder
caused by long-term PM2.5 exposure.
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| a |
School of Public Health, North China University of Science and Technology, Hebei 063210 China |
| b |
School of Psychology and Mental health, North China University of Science and Technology, Hebei 063210 China |
| c |
Hebei Key Laboratory of Organ Fibrosis Research, North China University of Science and Technology,
Hebei 063210 China |
| d |
Department of Endocrinology, North China University of Science and Technology Affiliated Hospital,
Hebei 063210 China |
| e |
Animal Experimental Center, North China University of Science and Technology, Hebei 063210 China |
* Corresponding author, E-mail: c-fuyuan@163.com, lishuangdwzx@ncst.edu.cn
Received 18 Jul 2024, Accepted 21 Feb 2025
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