Research articles
ScienceAsia 51 (2023): 1-8 |doi:
10.2306/scienceasia1513-1874.2023.020
In vitro antibacterial activity of panduratin A and its
derivatives against vancomycin-resistant Entercoccus faecium
clinical isolates
Kasarnchon Mek-yonga, Kodchaporn Jiangjamjitb, Chanita Napaswada, Anchalee Homkaewc,
Napason Chabangd, Tienthong Thongpanchangb, Padungsri Dubbsa, Sunhapas Soodvilaie,*
ABSTRACT: Discovery and development of antibiotics against vancomycin-resistant E. faecium (VREfm) is of high
priority. The aim of this study was to investigate the therapeutic potential of panduratin A, a bioactive compound
isolated from Boesenbergia rotunda, due to its antibacterial activity against Thai clinical isolates of VREfm. Susceptibility
testing of panduratin A using a microbroth dilution method revealed a minimum inhibitory concentration (MIC) of
2 ?g/ml against all 39 VREfm isolates tested. The antibacterial effect of panduratin A was bacteriostatic in most
isolates. The time-kill data revealed that panduratin A at 1?MIC inhibited bacterial growth for at least 24 h. The
inhibitory effect was more pronounced compared with 1?MIC linezolid, a standard antibiotic for VREfm treatment.
All VREfm isolates from this study exhibited vanA-type resistance supporting a crucial role of this type of vancomycin
resistance in Thailand. Additionally, the antibacterial activity of the modified chemical structure of panduratin A reveals
that a modification of the hydroxyl group(s) of panduratin A structure by either mono- or bis-alkylation abolished the
antibacterial activity against VREfm. The present study reveals a potential use for panduratin A in the treatment of, at
the least, vanA positive VREfm infection.
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| a |
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400 Thailand |
| b |
Department of Chemistry, Faculty of Science, Mahidol University, Bangkok 10400 Thailand |
| c |
Microbiological Unit, Central Laboratory and Blood Bank, Faculty of Medicine Vajira Hospital,
Navamindradhiraj University, Bangkok 10300 Thailand |
| d |
School of Bioinnovation and Bio-Based Product Intelligence, Faculty of Science, Mahidol University,
Bangkok 10400 Thailand |
| e |
Department of Physiology, Faculty of Science, Mahidol University, Bangkok 10400 Thailand |
* Corresponding author, E-mail: Sunhapas.soo@mahidol.ac.th
Received 9 Apr 2024, Accepted 10 Aug 2024
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