Research articles
ScienceAsia 51 (2023): 1-8 |doi:
10.2306/scienceasia1513-1874.2023.030
Daily intake of Lactobacillus paracasei (Lacticaseibacillus
paracasei) TISTR2593 ameliorated dyslipidemia and hepatic
steatosis in rats fed on high-cholesterol diets by modulating
lipid metabolism
Jaruwan Sitdhipola, Pennapa Chonpathompikunlerta,d,*, Thanaphol Thanagornyothina,
Neungnut Chaiyawana, Nasisorn Suksridechacina, Nutthaphong Phongnub, Wachirawadee Malakulb,c,*
ABSTRACT: Dietaryprobioticsupplementhasbeendocumentedtoeffectivelyamelioratehyperlipidemia and its related
diseases. Therefore, we aimed to study the dietary effect of Lactobacillus paracasei TISTR2593 (Lp) supplement on a
high-cholesterol diet (HCD)-induced hyperlipidemia and liver steatosis in rats, and to explore the possible underlying
mechanisms. The experiments were performed in 4 groups of rats, the control (receiving standard diet), and 3 treated
groups: HC, HC plus Lp (2?108 cfu/ml/day), and HC plus simvastatin (40 mg/kg/day). After 8 weeks of treatment,
blood and liver were collected for biochemical analysis and histopathological studies. The hepatic expressions of
proteins involved in lipid synthesis and uptake were analyzed by Western blotting. The oral administration of Lp
lowered the serum levels of total cholesterol, triglycerides, LDL-C, alanine aminotransferase (ALT), and aspartate
aminotransferase (AST), and increased serum HDL-C level in HCD-fed rats. Additionally, this probiotic reduced hepatic
triglycerides and cholesterol contents and improved histological characteristics of hepatic steatosis in HCD-fed rats.
Western blot analysis showed that the hepatic expressions of sterol regulatory element binding protein (SREBP)
1c, SREBP2, fatty acid synthase (FAS), stearoyl-CoA desaturase (SCD)-1, 3-hydroxy-3-methylglutaryl-CoA reductase
(HMGCR), and proprotein convertase subtilisin/kexin type 9 (PCSK9) were downregulated; while the expression of
low-density lipoprotein receptor (LDLR) was upregulated in the HC plus Lp-treated group. These results indicated
that daily consumption of Lp alleviated HCD-induced hyperlipidemia and hepatic steatosis in part by downregulating
proteins involving in lipogenesis and cholesterogenesis and modulating PCSK9- and LDLR-mediated hepatic LDL-C
uptake.
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| a |
Biodiversity Research Centre (BRC), Thailand Institute of Scientific and Technological Research (TISTR),
Pathum Thani 12120 Thailand |
| b |
Department of Physiology, Faculty of Medical Science, Naresuan University, Phitsanulok 65000 Thailand |
| c |
Centre of Excellence in Medical Biotechnology, Naresuan University, Phitsanulok 65000 Thailand |
| d |
Pre-Clinical and Clinical Research Service Unit (P2CRSU), A Division of Research and Development Group for
Bio-Industries, TISTR, Pathum Thani 12120 Thailand |
* Corresponding author, E-mail: wachirawadeema@nu.ac.th, pennapa@tistr.or.th
Received 25 Feb 2024, Accepted 26 Feb 2025
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