Research articles
ScienceAsia 50 (2024):ID 2024071 1-8 |doi:
10.2306/scienceasia1513-1874.2024.071
Bioethanol production from cassava starch using co-culture of
saccharolytic molds with Saccharomyces cerevisiae TISTR 5088
Timakorn Pimpisaia, Cherdsak Maneerattanarungroja,b, Engkarat Kingkaewa, Duangjai Ochaikula,b,*
ABSTRACT: Cassava serves as a cornerstone for sustainable bioethanol production in Thailand. The cassava root is
highly valuable, comprising 50?70% starch on a dry weight basis. Starch can be converted into fermentable sugars
through chemical or biological methods. This study focused on harnessing the enzymatic potential of saccharolytic
molds, specifically Aspergillus oryzae TISTR 3086 or Amylomyces rouxii TISTR 3182, to facilitate saccharification
of cassava starch, followed by fermentation using Saccharomyces cerevisiae TISTR 5088 for bioethanol production.
Among the various configurations, the co-culture of Amy. rouxii TISTR 3182 with S. cerevisiae TISTR 5088 was the
most effective, resulting in the highest ethanol yield. This finding was observed for both the separate hydrolysis
and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) processes. Impressively, the SSF
approach to ethanol productivity demonstrated superior results with an ethanol concentration of 25.4?0.3 g/l and an
ethanol productivity of 0.53 g/l/h (yield of 1.40 g/g reducing sugars), surpassing the SHF approach (25.4?0.7 g/l,
an ethanol productivity of 0.26 g/l/h, yield of 0.57 g/g reducing sugars) while also reducing the fermentation period.
Further investigations into optimizing the conditions for ethanol production were carried out using a co-culture of
Amy. rouxii TISTR 3182 with S. cerevisiae TISTR 5088 during SSF. This exploration revealed that employing 100 g/l
cassava starch and initiating fermentation with a medium pH of 6.0 led to the highest ethanol concentration at 48 h.
This process showed potential for ethanol production, harnessing the synergistic action of saccharolytic molds alongside
yeast.
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| a |
Department of Biology, School of Science, King Mongkut?s Institute of Technology Ladkrabang (KMITL),
Bangkok 10520 Thailand |
| b |
Bioenergy Research Unit, School of Science, King Mongkut?s Institute of Technology Ladkrabang (KMITL),
Bangkok 10520 Thailand |
* Corresponding author, E-mail: daungjai.oc@kmitl.ac.th
Received 0 0000, Accepted 11 Jun 2024
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