Research articles
ScienceAsia 50 (2024):ID 2024082 1-7 |doi:
10.2306/scienceasia1513-1874.2024.082
Prokaryotic expression of the Mycobacterium tuberculosis
PhoY2 gene and its diagnostic utility in various populations
infected with tuberculosis
Jingyan Zhanga, Qiangsen Zhongb, Xiaochun Wangb,*
ABSTRACT: Early and timely diagnosis is crucial for reducing the transmission of tuberculosis. In this study, we
constructed and expressed PhoY2, a persistent infection related antigen of Mycobacterium tuberculosis and evaluated
its potential as a diagnostic target through population experiments. The immunogenicity of the recombinant PhoY2
(rPhoY2) protein in M. tuberculosis infected individuals was evaluated using whole blood interferon-gamma (IFN-?)
release assay (WBIA) and serum specific antibody detection. Further, the diagnostic value, sensitivity, and specificity of
rPhoY2-WBIA and anti-rPhoY2 specific antibody in different M. tuberculosis infected populations were analyzed using
receiver operating characteristic. The results indicated that rPhoY2 can stimulate specific IFN-? production in active
tuberculosis (ATB) and latent tuberculosis infection (LTBI), levels of which were significantly higher than that in healthy
controls (HCs). The anti-rPhoY2 specific IgG levels of LTBI were significantly higher than those of ATB and HCs. The
cutoff value of rPhoY2-WBIA for diagnosing M. tuberculosis infection is 309.2 pg/ml with a sensitivity of 72.2% and a
specificity of 93.7%. The cutoff value of anti-rPhoY2 specific IgG for diagnosing LTBI is 0.1765 with 61.5% sensitivity
and 75% specificity. The agreement rate between rPhoY2-WBIA combined with specific antibodies and clinical diagnosis
for the three populations is 69.9%. In summary, the combination of rPhoY2-WBIA and specific antibody detection has
certain value in distinguishing different populations infected with M. tuberculosis, especially in excluding the infection
with high specificity. However, its sensitivity should be further enhanced for differentiating ATB and LTBI.
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| a |
Department of Clinical Laboratory, Affiliated Heping Hospital, Changzhi Medical College, 046000 China |
| b |
Department of Pathogen Biology, School of Medicine, Anhui University of Science and Technology, 232001 China |
* Corresponding author, E-mail: wxcvieri@126.com
Received 7 Oct 2023, Accepted 10 Jun 2024
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