Research articles
ScienceAsia 51 (2023): 1-8 |doi:
10.2306/scienceasia1513-1874.2023.019
Kinetin and indole-3-butyric acid for effective in vitro
propagation of the endangered Plectranthus phuluangensis
(Lamiaceae) and successful ISSR genetic fidelity test
Arpakorn Sakulsathaporna,*, Kanjana Jantarata, Chanram Roopkhamb
ABSTRACT: Plectranthus phuluangensis, a critically endangered plant belonging to the Lamiaceae family, is charac
terized by its unique herbal aroma and is endemic to the Phu Langka Forest in Nakhon Phanom province, Thailand.
This investigation aimed to establish an efficient in vitro micropropagation protocol for the rapid propagation of this
imperiled species utilizing nodal segment explants. The research successfully devised a protocol for the multiplication of
shoots, root initiation, and acclimatization. A half-strength Murashige and Skoog solid medium (?MS) was used, along
with different amounts of auxin (indole-3-butyric acid (IBA) and ?-naphthalene acetic acid (NAA)) and cytokinins (6
benzyladenine (BA), kinetin, and thidiazuron (TDZ)). The optimal medium for maximum shoot induction (9.01?1.10
shoots per explant) was at ?MS supplemented with 4 mg/l of kinetin. Moreover, the ?MS with 0.5 mg/l of kinetin
produced the longest shoots with an average length of 2.69?0.21 mm per shoot. Additionally, the introduction of
IBA and NAA led to the formation of adventitious roots. The maximum number of roots (7.35?0.63 roots per shoot)
was observed in ?MS media supplemented with 2.0 mg/l of IBA. After transferring to greenhouses, approximately
65% of the plantlets cultivated in vermiculite survived after 90 days. Furthermore, the ISSR molecular marker
analysis revealed no genetic variation between the propagated and mother plants, confirming the genetic fidelity of the
propagation method. This study represents the first account of in vitro propagation and genetic fidelity evaluation for
P. phuluangensis, presenting a feasible strategy for the preservation of this endangered species.
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| a |
Faculty of Interdisciplinary Studies, Khon Kaen University, Nong Khai 43000 Thailand |
| b |
Scientific Equipment and Research Division, Kasetsart University Research and Development Institute, Kasetsart
University, Bangkok 10900 Thailand |
* Corresponding author, E-mail: arpasa@kku.ac.th
Received 25 Jun 2023, Accepted 8 Jan 2025
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