Volume 49 Number 3

Development of β-glucan production from microorganisms as active ingredients in cosmeceutical products for skin youthfulness

Pornpoj Srisukchayakul^†, Tatsanee Werasura^†, iritorn Teeravet, Pradipha Pradapphai, Artitayapan Kannika, Thanchanok Muangman, Susakul Palakawong Na Ayudthaya, Chatrudee Suwannachart^*

 
ABSTRACT:     This study aims to select the potential microorganisms that contain high beta-glucan (?-glucan) and develop its cultivation, extraction, and purification methods from the selected microbe. The derived ?-glucan would be tested for its toxicity; the superoxide dismutase (SOD) activity as well as the production of collagen and elastin for development as an ingredient in the cosmetic industry would also be examined. Under this project, Saccharomyces cerevisiae TISTR 5027 is the supreme strain for ?-glucan production from 9 selected S. cerevisiae strains. The best medium for culturing S. cerevisiae TISTR 5027 is yeast mold broth (YM), supplemented with 0.5% peptone, agitated at 250?300 rpm, and incubated at 30?37 ?C for 60?72 h. After extraction, the derived ?-glucan at various concentrations was subjected to a toxicity test against human keratinocyte (HaCaT) cells and normal human dermal fibroblast (NHDF) cells. No adverse effect of cells was observed with the concentrations of ?-glucan of 3.13, 6.25, 12.50, 25.00, 50.00, and 100.00 ?g/ml. Interestingly, the concentrations of ?-glucan of 12.50 and 25.00 ?g/ml could activate the production of collagen in NHDF cells at 3.22?0.08, and 3.91?0.17%, respectively, while the production of collagen in untreated NHDF cells was 2.82?0.13%. Additionally, the same amounts of ?-glucan could enhance the production of elastin in NHDF cells at 86.12?1.62, and 84.11?6.02%, respectively, which were higher than those in untreated NHDF cells (78.71?3.60%).

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* Corresponding author, E-mail: chatrudee@tistr.or.th

Received 21 Jun 2023, Accepted 27 Mar 2024