Research articles
ScienceAsia (): 21-27 |doi:
10.2306/scienceasia1513-1874...021
Antifouling potential of Thalassia hemprichii extract
against growth of biofilm-forming bacteria
Fahruddin Fahruddin*, Eva Johannes, Zaraswati Dwyana
ABSTRACT: Many types of seagrass are known to produce active compounds, including those with the ability to
function as antimicrobials. This study focused on the potential of crude methanol extract from the seagrass Thalassia
hemprichii as an antimicrofouling agent. Seagrass T. hemprichii was macerated and then extracted with methanol. To
identify active components, biofilm bacterial inhibition assays were performed using the diffusion method. Isolates
of biofilm-forming bacteria were characterized and placed into eight groups. Bacterial inhibition assays demonstrated
that T. hemprichii extract was able to inhibit the growth of biofilm-forming bacteria, producing a 26 mm inhibition
zone. The effects of T. hemprichii extract was similar to chloramphenicol, which formed a 28 mm inhibition zone. No
inhibition zone was observed using methanol, the extraction solvent. The growth inhibitory properties of T. hemprichii
extract were further examined for each bacterial isolate. Inhibition zones produced by T. hemprichii extract using several
bacterial isolates were similar to chloramphenicol (27 mm): P1, 26 mm; P3, 27 mm; P4, 24 mm; P7, 26 mm; and P8,
26 mm. In contrast, T. hemprichii extract was less effective at inhibiting the growth of other bacterial isolates: P2,
21 mm; P5, 14 mm; and P6, 9 mm. Overall, our analysis demonstrates that T. hemprichii extract can inhibit the growth
of biofilm-forming bacteria, proving its potential as an antimicrofouling agent.
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Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Makassar,
Indonesia 90245 |
* Corresponding author, E-mail: fahruddin_science@unhas.ac.id
Received 17 Jul 2018, Accepted 28 Jan 2019
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