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Determination of gallic acid and rutin in extracts Cassiaÿalata and Andrographisÿpaniculata

BuranÿPhansawan^a,*, SupakornÿPongsabangpho^b

 
ABSTRACT:     This study determines gallic acid and rutin in ethanol, methanol, and acetic acid extracts of Cassia alata and Andrographis paniculata. A preliminary study using thin layer chromatography and 0.2% DPPH (2,2-diphenyl-1-picrylhydrazyl) spraying found that gallic acid was present in all three solvent extracts. Antioxidant components of all extractions were then analysed using high performance liquid chromatography with a C₁₈ reverse-phase column at a flow rate of 0.50ÿml/min and 25ÿ°C. The results showed high components of gallic acid and rutin in all extracts of C.ÿalata. The amounts of gallic acid found in C.ÿalata extracted with ethanol, methanol, and acetic acid were 366, 410, and 258ÿmg/g crude extract, respectively. Amounts of gallic acid found in A.ÿpaniculata extracted with ethanol, methanol, and acetic acid were 309.4, 351.2, and 309.1ÿmg/g crude extract, respectively. Rutin concentrations found in C.ÿalata extracted with ethanol, methanol and acetic acid were 194.4, 193.6, and 120.0ÿmg/g crude extract, respectively. The highest gallic acid level was found in C.ÿalata extracted with methanol, while the highest rutin levels were found in C.ÿalata extracted with ethanol and methanol.

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* Corresponding author, E-mail: buranphan@hotmail.com

Received 28 Jul 2014, Accepted 4 Dec 2014