Research articles
ScienceAsia (): 268-277 |doi:
10.2306/scienceasia1513-1874...268
Efficacy evaluation of the fibroblast-seeded collagen/chitosan scaffold on application in skin tissue engineering
Sirintip Intaraprasita, Atchariya Faikruab, Anuphan Sittichokechaiwutc, Jarupa Viyocha,*
ABSTRACT: There remains a need for dressings which aid the healing of chronically refractory wounds. To this end, we formulated and tested a collagen/chitosan scaffold supporting basic fibroblast growth factor (bFGF) producing cells. Collagen was blended with chitosan derived from crab shell (in ratio 7:3 of 3% weight of total polymer), crosslinked with glutaraldehyde, and then cast into a film. Tensile strength and elongation at break values of the scaffold was 8.5±0.1 MPa and 2.4±0.6%, respectively. The swelling degree was 77±4%, and the weight remaining after collagenase degradation for 1 month was 30.5±6.3%. Our scaffolds showed bio-compatibility because they encouraged attachment and proliferation of spindle-shaped human dermal fibroblasts (HDFs) which secreted surface-bound bFGF. Next, the fibroblast-seeded scaffold was prepared by seeding HDFs on the collagen/chitosan scaffold (5 × 105 cells/cm2) and further cultured for 7 days. These fibroblast-seeded scaffolds were used to dress full depth wounds in domestic pigs. The wounds with fibroblast-seeded scaffolds showed reduced swelling at day 7 and at all time points (7, 14, and 21 days), and re-epithelialization was faster than controls. Thus at day 21 of treatment, wounds treated with the fibroblast-seeded scaffold provided the highest percent re-epithelialization (69.7±9.6%), which was higher than untreated (49.9±6.3%) and cell-free scaffold treatment (54.3±6.9%). The wound tissue of the fibroblast-seeded scaffold treated group showed regular arrangement of host collagens and the rete ridge-like structure at the epidermal layer was also found at day 21. The results indicate the potential of the fibroblast-seeded scaffold for application in skin tissue engineering.
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| a |
Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences and Centre of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000 Thailand |
| b |
School of Medical Sciences, University of Phayao, Phayao 56000 Thailand |
| c |
Department of Preventive Dentistry, Faculty of Dentistry, Naresuan University, Phitsanulok 65000 Thailand |
* Corresponding author, E-mail: jarupav@nu.ac.th
Received 9 Nov 2011, Accepted 11 Sep 2012
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