Short reports
ScienceAsia (): 377-381 |doi:
10.2306/scienceasia1513-1874...377
Venom protein of the haematotoxic snakes Cryptelytrops albolabris, Calloselasma rhodostoma, and Daboia russelii siamensis
Orawan Khow, Pannipa Chulasugandha*, Narumol Pakmanee
ABSTRACT: The protein concentration and protein pattern of crude venoms of three major haematotoxic snakes of Thailand, Cryptelytrops albolabris (green pit viper), Calloselasma rhodostoma (Malayan pit viper), and Daboia russelii siamensis (Russell's viper), were studied. The protein concentrations of all lots of venoms studied were comparable. The chromatograms, from reversed phase high performance liquid chromatography, of C. albolabris venom and C. rhodostoma venom were similar but they were different from the chromatogram of D. r. siamensis venom. C. rhodostoma venom showed the highest number of protein spots on 2-dimensional gel electrophoresis (pH gradient 3–10), followed by C. albolabris venom and D. r. siamensis venom, respectively. The protein spots of C. rhodostoma venom were used as reference proteins in matching for similar proteins of haematotoxic snakes. C. albolabris venom showed more similar protein spots to C. rhodostoma venom than D. r. siamensis venom. The minimum coagulant dose could not be determined in D. r. siamensis venom.
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| Research and Development Department, Queen Saovabha Memorial Institute, Patumwan, Bangkok 10330 Thailand |
* Corresponding author, E-mail: pannipa_c@yahoo.com
Received 1 Dec 2010, Accepted 6 Sep 2011
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