Research articles
ScienceAsia 50 (2024):ID 2024102 1-8 |doi:
10.2306/scienceasia1513-1874.2024.102
Antioxidant and xanthine oxidase inhibitory activities of
rosmarinic acid-rich extract from perilla seed meal
Wachiraporn Tipsuwan, Komsak Pintha, Maitree Suttajit, Payungsak Tantipaiboonwong,
Piyawan Nuntaboon, Wittaya Chaiwangyen*
ABSTRACT: Hyperuricemia can lead to various diseases, including diabetes, renal disorders, and gout. Xanthine
oxidase (XO), a crucial enzyme, synthesizes uric acid from xanthine; and concurrently generates reactive oxygen
species, causing oxidative stress. This study aimed to validate the biological function of perilla seed meal (PSM), a
by-product of cold-pressed perilla oil, concerning phytochemical contents, antioxidants, and XO inhibitory activities.
PSM extraction employed 70% ethanol, and the solvent partition technique enriched the rosmarinic acid (RA)-rich
fraction. Total phenolic and flavonoid contents, along with antioxidant capabilities, were determined. The reducing
property of the PSM fraction was confirmed through the FRAP assay, while HPLC quantified RA. In addition, the
inhibitory efficiency of the PSM fraction against XO was investigated. The ethyl acetate (EA) fraction exhibited the
highest levels of phenolics, flavonoids, and RA. ABTS and DPPH assays revealed the most effective radical scavenging
by the EA fraction, with IC50 values of 6.51?0.11 ?g/l and 20.43?0.18 ?g/l, respectively. Moreover, the EA fraction
demonstrated the highest FRAP value of 3778.05?6.8 ?M Fe2+
/g. The XO inhibition of the EA fraction exhibited
significant inhibition compared to the ethanol extract. Mixed-type inhibition of XO was identified in PSM. Our results
suggest that PSM exhibits high phytochemical compounds, antioxidant capacities, and XO inhibition, meriting further
exploration for clinical applications.
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Division of Biochemistry, School of Medical Sciences, University of Phayao, Phayao 56000 Thailand |
* Corresponding author, E-mail: wittaya.ch@up.ac.th
Received 23 Jan 2024, Accepted 7 Sep 2024
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