Research articles
ScienceAsia 50 (2024):ID 2024110 1-10 |doi:
10.2306/scienceasia1513-1874.2024.110
Black rice bran-derived anthocyanins prevent H2O2-induced
oxidative stress and DNA damage in cholangiocytes through
activation of the Nrf2-NQO1 axis
Sasikamon Khophaia, Suwadee Chockchaisirib, Krajang Talabninc, James R. Ketudat Cairnsa, Chutima Talabnina,*
ABSTRACT: Anthocyanins are a group of hydrophilic flavonoids that exhibit various beneficial health effects, including
antioxidants and cancer prevention activities. Black rice bran has been demonstrated to be an excellent source of
anthocyanins. However, the efficacy of black rice bran-derived anthocyanin has never been demonstrated in cancer
prevention for oxidative stress-related cholangiocarcinoma (CCA). In this study, oxidative stress in cholangiocyte
cell lines (MMNK-1) was induced by hydrogen peroxide (H2O2
). The preventive effect of black rice bran-derived
anthocyanin (BBR-M-10) in H2O2
-stimulated cholangiocytes was studied through antioxidant capacity analysis,
measurement of intracellular ROS accumulation, and gene expression analysis at both the mRNA and protein levels.
Pretreatment with BBR-M-10 decreased oxidative stress in H2O2
-stimulated MMNK-1 cells by reduction of intracellular
ROS accumulation, leading to attenuated oxidative stress-induced cell death in H2O2
-stimulated MMNK-1 cells,
consistent with its up-regulation of survivin and down-regulations of cleaved caspase-3 and cleaved poly (ADP-ribose)
polymerase 1 (PARP1). Additionally, BBR-M-10 enhanced the ROS scavenging activity of the endogenous antioxidant
system by increasing the transcription level of Nrf2 and NQO1 in MMNK-1 cells exposed to H2O2
. Moreover, BBRM-10 reduced DNA damage of MMNK-1 cells stimulated by H2O2
through down-regulation of phospho-histone H2AX
(?H2AX). The cytoprotective properties against oxidative stress suggest their utility in preventing carcinogenic toxicity
during CCA development.
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| a |
School of Chemistry, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000 Thailand |
| b |
College of Allied Health Sciences, Suan Sunandha Rajabhat University, Samut Songkhram 75000 Thailand. |
| c |
School of Pathology, Institute of Medicine, Suranaree University of Technology, Nakhon Ratchasima 30000 Thailand |
* Corresponding author, E-mail: chutima.sub@sut.ac.th
Received 30 Dec 2023, Accepted 3 Aug 2024
|
