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Volume 34 Number 4 Volume 35 Number 1 Volume 35 Number 2

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Research articles

ScienceAsia 35 (2009): 37-41 |doi: 10.2306/scienceasia1513-1874.2009.35.037

Decolourization of pulp mill wastewater using thermotolerant white rot fungi

Sehanat Prasongsuka, Pongtharin Lotrakula, Tsuyoshi Imaib, Hunsa Punnapayaka,*

ABSTRACT:     Twenty isolates of white rot fungi were collected from several provinces in Thailand. Eight isolates could be cultured to test for thermotolerance and to screen for the presence of ligninolytic enzymes using Phanerochaete chrysosporium as a reference. Of the eight isolates, only three species Daedaleopsis sp., Schizophyllum commune PT, and S. commune SL were able to grow above 40 °C. All three species exhibited 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS)-oxidizing activity on ABTS agar plates. Only P. chrysosporium could oxidize manganese on MnCl2 agar plates. Both P. chrysosporium and Daedaleopsis sp. were able to decolourize wastewater on wastewater agar plates. All fungal cell suspensions tested decolourized wastewater No. 1 (pH 8.07, chemical oxygen demand (COD) 4347 mg/l) from the pulping process and wastewater No. 2 (pH 6.94, COD 4000 mg/l) from the pulping process combined with that from the paper recycling process. Daedaleopsis sp. and P. chrysosporium exhibited the highest ability to decolourize wastewater No. 1 (52%) and No. 2 (86%), respectively. Laccase activities were detected in the decolourized effluents and all fungi tested reduced the COD by 59–71% (No. 1) and 66–83% (No. 2).

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a Plant Biomass Utilization Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand
b Department of Civil and Environmental Engineering, Yamaguchi University, Ube, Japan

* Corresponding author, E-mail: phunsa@chula.ac.th

Received 23 May 2008, Accepted 20 Jan 2009