Research articles
ScienceAsia 50 (2024):ID 2024058 1-11 |doi:
10.2306/scienceasia1513-1874.2024.058
Differentiation potential of human amniotic fluid-derived
mesenchymal stem cells into hepatocyte-like cells
Napatsaree Nuttapasita, Sirinda Aungsuchawana, Suteera Narakornsaka,*, Waleephan Treebupachatsakula, Runchana Markmeea, Napatsorn Thongdia, Kantirat Yajaa, Peeraphan Pothacharoenb
ABSTRACT: Liver disease is recognized as a significant cause of death worldwide. Treatment methods involving tissue
and/or organ transplantation utilizing stem cells have emerged as an attractive alternative. Among the candidate
cells employed in regenerative medicine, mesenchymal stem cells (MSCs) have exhibited a promising potential as
beneficial cell source. Herein, the purpose of this study was to characterize the attributes of human amniotic fluidderived MSCs (hAF-MSCs) and induced hepatocyte-like cells. The results indicated that MSCs acquired from human
amniotic fluid cells uncovered proliferative potency along with the expression of MSCs markers. After hepatogenic
induction, the cells exhibited strong levels of expression of ALB, AFP, HNF4, and CYP1B1 genes, as well as ALB and
AFP proteins. In collaboration with the periodic staining associated with energy storage, the accumulation of glycogen
within the cytoplasm was observed after 21 days of cell cultivation. Additionally, the cells were capable of internalizing
indocyanine green into the cytoplasm. This reflected the potential detoxification of differentiated hepatocytes derived
from hAF-MSCs, which could support the use of MSCs in cell-based therapy.
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| a |
Department of Anatomy, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200 Thailand |
| b |
Thailand Excellence Center for Tissue Engineering and Stem Cells, Department of Biochemistry, Faculty of
Medicine, Chiang Mai University, Chiang Mai 50200 Thailand |
* Corresponding author, E-mail: suteera.n@cmu.ac.th
Received 29 Jul 2023, Accepted 4 Mar 2024
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