| Home  | About ScienceAsia  | Publication charge  | Advertise with us  | Subscription for printed version  | Contact us  
Editorial Board
Journal Policy
Instructions for Authors
Online submission
Author Login
Reviewer Login
Volume 50 Number 2
Volume 50 Number 1
Volume 49 Number 6
Volume 49 Number 5
Volume 49S Number 1
Volume 49 Number 4
Earlier issues
Volume  Number 

previous article next article

Research articles

ScienceAsia 50 (2024):ID 2024045 1-8 |doi: 10.2306/scienceasia1513-1874.2024.045


Development of β-glucan production from microorganisms as active ingredients in cosmeceutical products for skin youthfulness


Pornpoj Srisukchayakul, Tatsanee Werasura, iritorn Teeravet, Pradipha Pradapphai, Artitayapan Kannika, Thanchanok Muangman, Susakul Palakawong Na Ayudthaya, Chatrudee Suwannachart*

 
ABSTRACT:     This study aims to select the potential microorganisms that contain high beta-glucan (?-glucan) and develop its cultivation, extraction, and purification methods from the selected microbe. The derived ?-glucan would be tested for its toxicity; the superoxide dismutase (SOD) activity as well as the production of collagen and elastin for development as an ingredient in the cosmetic industry would also be examined. Under this project, Saccharomyces cerevisiae TISTR 5027 is the supreme strain for ?-glucan production from 9 selected S. cerevisiae strains. The best medium for culturing S. cerevisiae TISTR 5027 is yeast mold broth (YM), supplemented with 0.5% peptone, agitated at 250?300 rpm, and incubated at 30?37 ?C for 60?72 h. After extraction, the derived ?-glucan at various concentrations was subjected to a toxicity test against human keratinocyte (HaCaT) cells and normal human dermal fibroblast (NHDF) cells. No adverse effect of cells was observed with the concentrations of ?-glucan of 3.13, 6.25, 12.50, 25.00, 50.00, and 100.00 ?g/ml. Interestingly, the concentrations of ?-glucan of 12.50 and 25.00 ?g/ml could activate the production of collagen in NHDF cells at 3.22?0.08, and 3.91?0.17%, respectively, while the production of collagen in untreated NHDF cells was 2.82?0.13%. Additionally, the same amounts of ?-glucan could enhance the production of elastin in NHDF cells at 86.12?1.62, and 84.11?6.02%, respectively, which were higher than those in untreated NHDF cells (78.71?3.60%).

Download PDF

0 Downloads 113 Views


a Thailand Institute of Scientific and Technological Research (TISTR), Pathum Thani 12120 Thailand

* Corresponding author, E-mail: chatrudee@tistr.or.th

Received 21 Jun 2023, Accepted 27 Mar 2024