Research articles
ScienceAsia 50 (2024):ID 2024052 1-8 |doi:
10.2306/scienceasia1513-1874.2024.052
Development of the HPLC-DAD method for chromone
determination and standardization in Harrisonia perforata
(Blanco) Merr. and Thai herbal Ya-Ha-Rak formulation
Supawan Wechprakhona, Bungorn Sripanidkulchaia, Naphapak Jaipakdeea,b, Peera Tabboona,b,*
ABSTRACT: Harrisonia perforata (Blanco) Merr. (H. perforata) is a plant material used in the Ya-Ha-Rak formula for
the treatment of fever in COVID-19 patients in Thailand and contains two chromones as major active compounds,
namely O-methylalloptaeroxylin (OML) and peucinin-7-methyl ether (P7ME). In Thailand, however, there is currently
no standard quality control method available for this plant. This study aims to develop and validate analytical methods
to measure levels of OML and P7ME in samples including branches, roots, and extracts of H. perforata, and Ya-Ha-Rak
formulations. In the development process, a number of columns and elution conditions to determine OML and P7ME
in the Ya-Ha-Rak formulations and H. perforata raw materials were investigated. Ideal conditions, including C18, highpurity silica at 4.6?150 mm, 3.5 ?m particle size, controlling the column oven at 40 ?C, and eluting with an acidic
mobile phase, significantly improved the separation of OML and P7ME from other elutes in the chromatogram with
good suitability parameters. The validation method gave good values for the correlation coefficient (R
2 = 0.9999) for
both compounds. The limits of detection (LOD) and quantification (LOQ) were in the ranges of 1.31 and 1.03 ?g/ml
and 4.37 and 3.45 ?g/ml, respectively. The recovery percentage fell between 99.97 and 109.75%, and the RSD value of
inter-day and intra-day determinations was under 2.0%. The validated method was successfully applied to the analysis
of real Ya-Ha-Rak and H. perforata raw material samples from various areas in Thailand.
Download PDF
0 Downloads 485 Views
| a |
Center for Research and Development of Herbal Health Products, Khon Kaen University, Khon Kaen 40002 Thailand |
| b |
Division of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Khon Kaen University,
Khon Kaen 40002 Thailand |
* Corresponding author, E-mail: peerata@kku.ac.th
Received 19 Jun 2023, Accepted 10 Apr 2024
|
