Indobet11
   | Home  | About ScienceAsia  | Publication charge  | Advertise with us  | Subscription for printed version  | Contact us  
Editorial Board
Journal Policy
Instructions for Authors
Online submission
Author Login
Reviewer Login
Volume 50 Number 2
Volume 50 Number 1
Volume 49 Number 6
Volume 49 Number 5
Volume 49S Number 1
Volume 49 Number 4
Earlier issues
https://nagaempire7.xyz/ https://empire88t.com/ https://botak-empire5.com/ https://uncleempire19.xyz/ https://indobet11j.com/ https://mukapoker1.lol/ https://mukacasinoc.com/ https://138.197.6.28 https://138.68.168.98 https://152.42.182.182/ https://planetaryconquest.com/ https://internationalpeaceday.com/ https://weidenfeldlaw.com/ https://notariamc.com/ https://inetskate.com/ https://newdatingway.com/ https://korem083.tniad.mil.id/shop/slot-100-perak/ https://korem083.tniad.mil.id/product/slot-qris/ https://korem083.tniad.mil.id/store/bet-200-perak/ https://abkpmi.ac.id/wp-content/uploads/products/slot-qris-10k/
https://www.arabiaphone.com/ https://www.benchmarcsystems.com/ https://www.dndock.com/ https://www.virtuallytheoffice.com/ https://www.mikechomes.com/ https://www.studioelpizo.com/ https://www.musicrebellion.com/ https://www.patmcgann.com/ https://www.hotel-gufler.com/ https://www.aegeaneating.com/ https://www.peterclementbooks.com/ https://www.mahshidabbasi.com/ https://www.elizabethtoop.com/ https://www.stonedeadforever.com/ https://www.longislandsites.com/ https://www.stevenmaloff.com/ https://www.viananaturalhealing.com/ https://www.dascomputers.com/ https://www.systemf3.com/ https://www.visitguanacaste.com/ https://www.independentnepa.com/ https://www.theobooks.org/ https://www.charlieshd.com/ https://www.robertcatkinson.com/ https://www.lechevart.com/ https://www.conkerco.com/ https://www.postgal.com/ https://www.drharoldlong.com/ https://www.blackmenvent.com/ https://www.paranormalresearchonline.com/ http://www.joshkrischer.com/ https://www.riccmho.org/ https://www.iflorabella.com/ https://www.marcellatracco.com/ https://www.juliehutchinson.com/ https://www.fiestadocumentary.com/ https://prakerja.cybersacademy.id/uk/sgacor https://jurnal-fastek.ikopin.ac.id/styles/ http://rodaberita.id/wp-content/botakempire/ https://rodaberita.id/wp-content/empire88/ https://rodaberita.id/wp-content/uncleempire/ https://univ.ikopin.ac.id/wp-content/uncleempire/ https://univ.ikopin.ac.id/wp-content/indobet11/ https://univ.ikopin.ac.id/wp-content/empire88/ https://univ.ikopin.ac.id/wp-content/botak-empire/ https://rodaberita.id/wp-content/indobet11 Botak Empire
Maxwin Botak Empire
Botak Empire Scatter
Daftar Botak Empire
Botak Empire Rekomendasi
Botak Empire Menang
Botak Empire Akun Pro
Scatter Botak Empire
Botak Empire Deposit Receh
Empire88 Slot 4D
Botak Empire Alternatif
Volume  Number 

previous article next article

Research articles

ScienceAsia 50 (2024):ID 2024024 1-8 |doi: 10.2306/scienceasia1513-1874.2024.024


Enhanced productions of poly-3-hydroxybutyrate and glycogen in cyanobacterium Synechocystis sp. PCC 6803 by disrupting related biosynthetic pathways under phosphorus deprivation


Janine Kaewbai-ngama,b, Tanakarn Monshupaneea,*

 
ABSTRACT:     Under normal growth (NORMAL) condition with adequate supply of phosphorus and nitrogen, the wellstudied cyanobacterium Synechocystis wild type (WT) produced a high biomass level but subtle contents of the two biopolymers: poly-3-hydroxybutyrate (PHB) and glycogen. In contrast, under nitrogen deprivation, growth of WT was terminated, while the accumulations of PHB and glycogen were increased. Thus, a strain and a cultivation strategy that simultaneously allows both biomass production and the increased storages of the two biopolymers are desirable. Here, under phosphorus deprivation (-P), WT grew and still produced biomass at 850 mg/l (accounting for 62% of the biomass level obtained under NORMAL cultivation) with slightly increased contents of PHB and glycogen at 0.5% and 5.5% (w/w DW), respectively. Under -P, the ?G mutant with the inactivated glycogen synthesis produced biomass at 751 mg/l and enhanced PHB accumulation to 4.9% (w/w DW), corresponding to PHB production of 36 mg/l. The ?HP mutant with the inactivation of both PHB and hydrogen-gas syntheses under -P produced biomass at 713 mg/l and increased glycogen storage to 14.6% (w/w DW), corresponding to glycogen production of 118 mg/l. The significantly reduced levels of both chlorophyll and phycobilisomes (the main photosynthetic protein complex) found in ?G and ?HP mutants under -P suggested that photosynthetic proteins were degraded into amino acids which might subsequently be used for PHB and glycogen syntheses. The results indicated that the metabolic engineering combined with -P cultivation concurrently allowed cell growth and increased the production of two biopolymers in the studied Synechocystis.

Download PDF

0 Downloads 289 Views


a Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330 Thailand
b Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok 10330 Thailand

* Corresponding author, E-mail: tanakarn.m@chula.ac.th

Received 16 Jun 2023, Accepted 14 Dec 2023