Research articles
ScienceAsia 50 (2024):ID 2024018 1-8 |doi:
10.2306/scienceasia1513-1874.2024.018
Isolation and pathogenicity of Ralstonia solanacearum in
tomato and potential of phage BHDTSo81 against the
pathogen
Nguyen M. Thiena,b, Le T.T. Tiena,b, Vo T. Phuca,b, To H. Ngoca,b, Nam Vob,c,d, Hoang Duc Nguyenb,c, Tu Q. Vinha,b, Hoang A. Hoanga,b,*
ABSTRACT: The bacterial wilt disease in tomato caused by Ralstonia solanacearum results in harvest losses of up to
90% and significant economic loss to farmers. In this study, 11 R. solanacearum strains were isolated in the largest
tomato-growing province in Vietnam. These strains belonged to 3 different groups, and 9/11 strains showed different
disease rates in experimental tomato plants. A BHDTSo81 phage specific to R. solanacearum was isolated from a
soil sample. Morphological analysis indicated that BHDTSo81 had podovirus morphology and was classified into
the Autographiviridae family. The latent period and burst size of BHDTSo81 were calculated to be approximately
145 min and 8.6?1.8 virions per infected cell, respectively. In a test of 26 bacterial strains, BHDTSo81 infected 7/11
R. solanacearum strains, while none of the other bacteria tested were susceptible to the phages. R. solanacearum was
also challenged in vitro and was inactivated by BHDTSo81 for 40 h in broth. The genome of BHDTSo81 is 41,296 bp long
with a total GC content of 63% and contains 46 predicted protein-encoding CDSs (coding sequences). No virulence
factor or antibiotic resistance gene was found in the genome. Thus, the initial characteristics of phage BHDTSo81
indicate its potential utility as a control agent against R. solanacearum.
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a |
Faculty of Chemical Engineering, Ho Chi Minh City University of Technology, Ho Chi Minh City 700000 Vietnam |
b |
Vietnam National University Ho Chi Minh City, Thu Duc, Ho Chi Minh City 700000 Vietnam |
c |
Center for Bioscience and Biotechnology, University of Science, Ho Chi Minh City 700000 Vietnam |
d |
Laboratory of Molecular Biotechnology, University of Science, Ho Chi Minh City 700000 Vietnam |
* Corresponding author, E-mail: hoang.a.hoang@hcmut.edu.vn
Received 28 Apr 2023, Accepted 3 Dec 2023
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