Research articles
ScienceAsia 50 (2024):ID 2024079 1-8 |doi:
10.2306/scienceasia1513-1874.2024.079
Assessing the defense role of phenoloxidase in Spodoptera
litura (Fab.) challenged with entomopathogenic fungi
Sonam Rajputa,b, Sachin S. Suroshea,*, Purnamasi R. Yadavb, Anoop Kumarc
ABSTRACT: The study was conducted to evaluate the bioefficacy of two entomopathogenic fungi (EPFs), Metarhizium
anisopliae (Metchnikoff) Sorok (Ma-NCIPM) and Beauveria bassiana (Balsamo) Vuill. (Bb-NCIPM), and the effect of
these EPFs on the host defense related enzyme, phenoloxidase (PO) in Spodoptera litura (Fabricius) using two different
bioassay methods, namely larval immersion and topical spray. The results revealed that the bioefficacy of Ma-NCIPM
and Bb-NCIPM at 1?108
spores/ml against the third instar larvae of S. litura significantly differed. Ma-NCIPM recorded
the highest (68?0.49%) mortality, and Bb-NCIPM showed the lowest (56?0.9%) mortality with larval immersion
method at 168 h of application. The highest mortality was reported by Ma-NCIPM (98?2.62%), followed by the BbNCIPM (70?0.32%) with topical spray method at 168 h after treatment. Compared to control, the LT50 of Ma-NCIPM
and Bb-NCIPM diminished by 3.41 and 1.03 folds, respectively, through larval immersion method. However, through
topical spray method, LT50 of Ma-NCIPM and Bb-NCIPM diminished by 4.05 and 1.2 folds, respectively. The level of
PO specific activity in the host, S. litura larvae also fluctuated by the exposure of Ma-NCIPM and Bb-NCIPM compared
to the control.
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| a |
Division of Entomology, ICAR-Indian Agricultural Research Institute, Pusa, New Delhi 110012 India |
| b |
Zoology Department, D A V College (C C S University, Meerut), Muzaffarnagar, UP 251001 India |
| c |
ICAR-National Research Centre for Integrated Pest Management, New Delhi 110012 India |
* Corresponding author, E-mail: sachinsuroshe@gmail.com, sachinsuroshe@yahoo.com
Received 28 Feb 2023, Accepted 16 May 2024
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