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Research articles

ScienceAsia 49 (2023): 595-602 |doi: 10.2306/scienceasia1513-1874.2023.058

A novel long noncoding RNA, AC005062.1, acts as an oncogene in colorectal cancer cell by regulating MACC1

Zhixiang Li*, Qiang Xie, Binbin Wang, Dajun Yu, Chunfang Liu, Kai Wang, Ligong Zhang, Chao Zhu

ABSTRACT:     A novel long noncoding RNA (lncRNA), AC005062.1, has shown an abnormal expression in bladder cancer and small cell lung cancer; however, it has never been studied in colorectal cancer (CRC). In this study, we explored the expression and role of AC005062.1 in CRC. The expression of AC005062.1 in CRC tissues and cell lines was determined using quantitative real-time PCR (qRT-PCR). In vitro experiments such as CCK-8 assay, flow cytometry, wound scratch test, and transwell assay were performed in HT-29 cells after knockdown of AC005062.1. Our results showed that AC005062.1 was significantly up-regulated in CRC tissues and positively correlated with advanced clinical stage, larger tumor size, lymphatic metastasis, distant metastasis, and vascular invasion. Knockdown of AC005062.1 in HT-29 cells significantly inhibited cell proliferation, migration, and invasion and promoted cell apoptosis and G1 arrest. mRNA and protein expression levels of Metastasis Associated in Colon Cancer 1 (MACC1), a neighboring gene of AC005062.1, were decreased after the knockdown of AC005062.1. Our study demonstrated that AC005062.1, which acts as an oncogene, may be involved in CRC tumorigenesis and progression by regulating MACC1.

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a Department of Tumor Surgery, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233004 China

* Corresponding author, E-mail: lizhixiang.118@163.com

Received 2 Dec 2022, Accepted 8 May 2023