Research articles
ScienceAsia 50 (2024):ID 2024021 1-10 |doi:
10.2306/scienceasia1513-1874.2024.021
MicroRNA-153-3p promotes hepatocellular carcinoma
progression by modulating SPRY2 expression and the
MAPK/ERK signalling pathway
Peihua Yuana, Zhiling Zhaoa, Wenjie Hua, Hongyang Lia, Jieying Liub,*, Luying Liua,*
ABSTRACT: Hepatocellular carcinoma (HCC) is the most common primary liver cancer and among the most prevalent
digestive system cancers. MicroRNAs (miRNAs) have been linked to multiple cellular processes, including cellular
proliferation, differentiation, and cancer development. Although miRNA-153-3p participates in various malignancies,
its role in HCC and its underlying mechanisms remain unclear. In this study, we investigated the expression and
molecular mechanisms of miRNA-153-3p in HCC. miRNA-153-3p and SPRY2 expression was evaluated in human
tissues and cell lines using quantitative PCR and western blotting. The cell proliferation, migration, and invasion
capacities were assessed using Cell Counting Kit-8 and Transwell assays. The cell cycle and apoptosis were analysed
using flow cytometry, and the relationship between miRNA-153-3p and SPRY2 was confirmed using a dual-luciferase
reporter assay. miRNA-153-3p was upregulated, whereas SPRY2 was downregulated in HCC tissues compared with
para-carcinoma tissues. Overexpression of miRNA-153-3p promotes HCC proliferation, migration, and invasion by
targeting SPRY2 and mitogen-activated protein kinase/extracellular signal-regulated kinase signalling pathway-related
proteins. It was also observed that SPRY2 overexpression partially rescued the effects of miRNA-153-3p. This study
provides an experimental reference for identifying molecular targets for HCC therapy
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a |
Department of Pathology, Binzhou Medical University, Yantai, Shandong 264003 China |
b |
Department of Physiology, Binzhou Medical University, Yantai, Shandong 264003 China |
* Corresponding author, E-mail: liujybz@126.com, llixq@126.com
Received 19 Sep 2022, Accepted 10 Dec 2023
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